GFP antibody

Catalog No. ABIN5691122

This anti-GFP antibody is a Rabbit Polyclonal antibody detecting GFP in WB, ELISA and IF. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (ABIN5691122)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-GFP Antibody

Specificity: The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.

Cross-Reactivity (Details): Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.

Purification: Immunoaffinity purified

Immunogen: The anti-GFP antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with highly purified native GFP derived from Aequorea victoria jellyfish.

Application Details

Application Notes: The antibody is suitable for the detection of GFP and its variants by Western blotting, ELISA and Immunocytochemistry. Optimal antibody dilutions for the different applications should be determined by titration. Recommended dilution for ELISA: 1:5,000 to 1:25,000. For immunoblotting a dilution of 1:2,000 to 1:10,000 is recommended. For immunocytochemistry the antiserum can be diluted 1:100 to 1:250.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 μL 1 mg/mL immunoaffinity purified polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C, or in small aliquots at -20°C.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: Green Fluorescent Protein

Background: Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.

UniProt: P42212